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pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre
(Plasmid #134308)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134308 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 5065
  • Total vector size (bp) 10942
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Synaptophysin
  • Species
    M. musculus (mouse), R. norvegicus (rat)
  • Insert Size (bp)
    2361
  • GenBank ID
    NM_009305
  • Entrez Gene
    Syp (a.k.a. A230093K24Rik, Syn, p38)
  • Promoter CAG
  • Tag / Fusion Protein
    • TdTomato (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mfe1 (destroyed during cloning)
  • 3′ cloning site Xma1 (not destroyed)
  • 5′ sequencing primer TTCGGCTTCTGGCGTGTG
  • 3′ sequencing primer CGGCTTCGGCCAGTAACG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Cre-ER
  • Alt name
    OHT-Cre
  • Species
    Synthetic
  • Insert Size (bp)
    2929
  • Promoter CAG

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstX1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer TACATGTGTTTAGTCGAGG
  • 3′ sequencing primer TTATGTTTCAGGTTCAGGG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre was a gift from Elly Nedivi (Addgene plasmid # 134308 ; http://n2t.net/addgene:134308 ; RRID:Addgene_134308)

  • For your References section:

    CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses for Stabilization by Facilitating PSD95 Recruitment. Subramanian J, Michel K, Benoit M, Nedivi E. Cell Rep. 2019 Aug 6;28(6):1584-1595.e5. doi: 10.1016/j.celrep.2019.07.012. 10.1016/j.celrep.2019.07.012 PubMed 31390571
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