Donor_eGP2AP_RC
(Plasmid #133784)

• Purpose: (Empty Backbone) Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.
• Depositing Lab: Sriram Kosuri
• Publication: Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. doi: 10.1016/j.cels.2020.05.011. Epub 2020 Jun 29.

Backbone

• Vector backbone: pUC57
• Backbone size (bp): 4465
• Modifications to backbone: SpeI and MluI cloning sites between bGH pA sequence and BxB1 attB site. Insertion of promoterless eGFP-P2A-Puro and bGH pA sequence downstream of BxB1 site. Also introduction of a lox 511 site downstream that was not used.
• Vector type: Mammalian Expression
• Selectable markers: Puromycin
• Tag / Fusion Protein:
  • eGFP-P2A-Puro

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: backbone grown at 37 °C. Downstream of library cloning, most growth performed at 30 °C.
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pjd_donor_egp2ap_rc (1).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry