pMXs-Klf4
(Plasmid #13370)

• Purpose: Retroviral expression of mouse Klf4
• Depositing Lab: Shinya Yamanaka
• Publication: Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Backbone

• Vector backbone: pMXs
• Backbone size w/o insert (bp): 4600
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kruppel-like factor 4 (gut)
• Alt name: Klf4
• Species: M. musculus (mouse)
• Insert Size (bp): 1425
• Mutation: None
• Entrez Gene: Klf4 (a.k.a. EZF, Gklf, Zie)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pMX-S1811 (5'-GACGGCATCGCAGCTTGGATACAC-3')
• 3′ sequencing primer: LNCX-3 (5'-ACCTACAGGTGGGGTCTTTCATTCCC-3')

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
• Articles Citing this Plasmid:
  • 82 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.

Mouse Klf4 includes G372A, A459C, G527C, G633C, G1021C, C1305G and T1368C mutations.
All of substitutions can be found in EST database of NCBI. So we believe that these differences are caused by SNPs.
As we have shown in the previous our papers, these changes do not affect biological functions of the transcription factors during reprogramming.