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Addgene

Actin.CIB.GFP
(Plasmid #133618)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133618 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    phCMV - GFP
  • Backbone manufacturer
    Genelantis
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    betaActin - CIB
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Insert Size (bp)
    1701
  • Promoter cmv
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site xhoI (unknown if destroyed)
  • 3′ cloning site hindIII (unknown if destroyed)
  • 5′ sequencing primer cmvF
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/857706v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Actin.CIB.GFP was a gift from Robert Hughes (Addgene plasmid # 133618 ; http://n2t.net/addgene:133618 ; RRID:Addgene_133618)

  • For your References section:

    CofActor: A light- and stress-gated optogenetic clustering tool to study disease-associated cytoskeletal dynamics in living cells. Salem FB, Bunner WP, Prabhu VV, Kuyateh AB, O'Bryant CT, Murashov AK, Szatmari EM, Hughes RM. J Biol Chem. 2020 Aug 7;295(32):11231-11245. doi: 10.1074/jbc.RA119.012427. Epub 2020 May 18. 10.1074/jbc.RA119.012427 PubMed 32424038
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