SpyTag003-mKate2
(Plasmid #133452)

• Purpose: Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm
• Depositing Lab: Mark Howarth
• Publication: Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 10.1073/pnas.1909653116.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6024
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpyTag003-mKate2
• Species: Synthetic
• Insert Size (bp): 807
• Promoter: T7
• Tags / Fusion Proteins:
  • GSGSGS linker (C terminal on insert)
  • His6 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry