KE-005
(Plasmid
#133440)
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PurposeHuman elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133440 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET21 helF4HA1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEIF4A1
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SpeciesH. sapiens (human)
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Insert Size (bp)1218
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Entrez GeneEIF4A1 (a.k.a. DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: HsCD00483422.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KE-005 was a gift from Gerhard Wagner (Addgene plasmid # 133440 ; http://n2t.net/addgene:133440 ; RRID:Addgene_133440)
