pGAPZa-STE2-1D4
(Plasmid
#133422)
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PurposeMutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGAPZalpha
- Backbone size w/o insert (bp) 3100
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSTE2
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)951
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MutationC59S, C252A
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Entrez GeneSTE2 (a.k.a. YFL026W)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: ScCD00063804.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGAPZa-STE2-1D4 was a gift from Gerhard Wagner (Addgene plasmid # 133422 ; http://n2t.net/addgene:133422 ; RRID:Addgene_133422)
