pIRES:mTREK-1(K271Q)
(Plasmid
#133273)
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PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133273 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRES2-EGFP
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Vector typeMammalian Expression, Yeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePotassium channel subfamily K member 2 (KCNK2, TREK-1)
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SpeciesM. musculus (mouse)
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MutationK271Q
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Entrez GeneKcnk2 (a.k.a. A430027H14Rik, TREK-1)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV F
- 3′ sequencing primer CMV R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRES:mTREK-1(K271Q) was a gift from Dan Minor (Addgene plasmid # 133273 ; http://n2t.net/addgene:133273 ; RRID:Addgene_133273) -
For your References section:
K2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site. Lolicato M, Arrigoni C, Mori T, Sekioka Y, Bryant C, Clark KA, Minor DL Jr. Nature. 2017 Jul 20;547(7663):364-368. doi: 10.1038/nature22988. Epub 2017 Jul 10. 10.1038/nature22988 PubMed 28693035
