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Addgene

pMK1253
(Plasmid #133058)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133058 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSicoR (pMK1200)
  • Backbone size w/o insert (bp) 7817
  • Total vector size (bp) 8219
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    mClover2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tau.K18(LM)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    402
  • Promoter EF1A
  • Tag / Fusion Protein
    • mClover2

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMK1253 was a gift from Martin Kampmann (Addgene plasmid # 133058 ; http://n2t.net/addgene:133058 ; RRID:Addgene_133058)

  • For your References section:

    Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation. Chen JJ, Nathaniel DL, Raghavan P, Nelson M, Tian R, Tse E, Hong JY, See SK, Mok SA, Hein MY, Southworth DR, Grinberg LT, Gestwicki JE, Leonetti MD, Kampmann M. J Biol Chem. 2019 Oct 2. pii: RA119.009432. doi: 10.1074/jbc.RA119.009432. 10.1074/jbc.RA119.009432 PubMed 31578281
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