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pU6-pegRNA-GG-acceptor Citations (60)

Originally described in: Search-and-replace genome editing without double-strand breaks or donor DNA.
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. PubMed Journal

Articles Citing pU6-pegRNA-GG-acceptor

Articles
Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures. Billon P, Nambiar TS, Hayward SB, Zafra MP, Schatoff EM, Oshima K, Dunbar A, Breinig M, Park YC, Ryu HS, Tschaharganeh DF, Levine RL, Baer R, Ferrando A, Dow LE, Ciccia A. Cell Rep. 2020 Mar 10;30(10):3280-3295.e6. doi: 10.1016/j.celrep.2020.02.068. PubMed

Associated Plasmids
Highly Parallel Profiling of Cas9 Variant Specificity. Schmid-Burgk JL, Gao L, Li D, Gardner Z, Strecker J, Lash B, Zhang F. Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023. PubMed

Associated Plasmids
CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grunewald J, Joung JK. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. PubMed

Associated Plasmids
Unbiased investigation of specificities of prime editing systems in human cells. Kim DY, Moon SB, Ko JH, Kim YS, Kim D. Nucleic Acids Res. 2020 Oct 9;48(18):10576-10589. doi: 10.1093/nar/gkaa764. PubMed
A web tool for the design of prime-editing guide RNAs. Chow RD, Chen JS, Shen J, Chen S. Nat Biomed Eng. 2021 Feb;5(2):190-194. doi: 10.1038/s41551-020-00622-8. Epub 2020 Sep 28. PubMed
Prime editing for functional repair in patient-derived disease models. Schene IF, Joore IP, Oka R, Mokry M, van Vugt AHM, van Boxtel R, van der Doef HPJ, van der Laan LJW, Verstegen MMA, van Hasselt PM, Nieuwenhuis EES, Fuchs SA. Nat Commun. 2020 Oct 23;11(1):5352. doi: 10.1038/s41467-020-19136-7. PubMed
PrimeDesign software for rapid and simplified design of prime editing guide RNAs. Hsu JY, Grunewald J, Szalay R, Shih J, Anzalone AV, Lam KC, Shen MW, Petri K, Liu DR, Joung JK, Pinello L. Nat Commun. 2021 Feb 15;12(1):1034. doi: 10.1038/s41467-021-21337-7. PubMed
Engineered Prime Editors with PAM flexibility. Kweon J, Yoon JK, Jang AH, Shin HR, See JE, Jang G, Kim JI, Kim Y. Mol Ther. 2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 10.1016/j.ymthe.2021.02.022. PubMed

Associated Plasmids
PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants. Siegner SM, Karasu ME, Schroder MS, Kontarakis Z, Corn JE. BMC Bioinformatics. 2021 Mar 2;22(1):101. doi: 10.1186/s12859-021-04034-6. PubMed
Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing. Chemello F, Chai AC, Li H, Rodriguez-Caycedo C, Sanchez-Ortiz E, Atmanli A, Mireault AA, Liu N, Bassel-Duby R, Olson EN. Sci Adv. 2021 Apr 30;7(18). pii: 7/18/eabg4910. doi: 10.1126/sciadv.abg4910. Print 2021 Apr. PubMed
Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor. Park SJ, Jeong TY, Shin SK, Yoon DE, Lim SY, Kim SP, Choi J, Lee H, Hong JI, Ahn J, Seong JK, Kim K. Genome Biol. 2021 Jun 3;22(1):170. doi: 10.1186/s13059-021-02389-w. PubMed

Associated Plasmids
Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids. Geurts MH, de Poel E, Pleguezuelos-Manzano C, Oka R, Carrillo L, Andersson-Rolf A, Boretto M, Brunsveld JE, van Boxtel R, Beekman JM, Clevers H. Life Sci Alliance. 2021 Aug 9;4(10). pii: 4/10/e202000940. doi: 10.26508/lsa.202000940. Print 2021 Oct. PubMed
Precise genomic deletions using paired prime editing. Choi J, Chen W, Suiter CC, Lee C, Chardon FM, Yang W, Leith A, Daza RM, Martin B, Shendure J. Nat Biotechnol. 2021 Oct 14. pii: 10.1038/s41587-021-01025-z. doi: 10.1038/s41587-021-01025-z. PubMed

Associated Plasmids
Deletion and replacement of long genomic sequences using prime editing. Jiang T, Zhang XO, Weng Z, Xue W. Nat Biotechnol. 2021 Oct 14. pii: 10.1038/s41587-021-01026-y. doi: 10.1038/s41587-021-01026-y. PubMed

Associated Plasmids
Modelling of primary ciliary dyskinesia using patient-derived airway organoids. van der Vaart J, Bottinger L, Geurts MH, van de Wetering WJ, Knoops K, Sachs N, Begthel H, Korving J, Lopez-Iglesias C, Peters PJ, Eitan K, Gileles-Hillel A, Clevers H. EMBO Rep. 2021 Dec 6;22(12):e52058. doi: 10.15252/embr.202052058. Epub 2021 Oct 25. PubMed
piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition. Wolff JH, Haldrup J, Thomsen EA, Andersen S, Mikkelsen JG. Front Genome Ed. 2021 Nov 12;3:786893. doi: 10.3389/fgeed.2021.786893. eCollection 2021. PubMed

Associated Plasmids
Citric acid/beta-alanine carbon dots as a novel tool for delivery of plasmid DNA into E. coli cells. Pandey A, Devkota A, Sigdel A, Yadegari Z, Dumenyo K, Taheri A. Sci Rep. 2021 Dec 14;11(1):23964. doi: 10.1038/s41598-021-03437-y. PubMed
Insertion of the Icelandic Mutation (A673T) by Prime Editing: A Potential Preventive Treatment for Familial and Sporadic Alzheimer's Disease. Tremblay G, Rousseau J, Mbakam CH, Tremblay JP. CRISPR J. 2022 Feb;5(1):109-122. doi: 10.1089/crispr.2021.0085. Epub 2022 Feb 7. PubMed
Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair. Ferreira da Silva J, Oliveira GP, Arasa-Verge EA, Kagiou C, Moretton A, Timelthaler G, Jiricny J, Loizou JI. Nat Commun. 2022 Feb 9;13(1):760. doi: 10.1038/s41467-022-28442-1. PubMed
ABIN1 is a signal-induced autophagy receptor that attenuates NF-kappaB activation by recognizing linear ubiquitin chains. Shinkawa Y, Imami K, Fuseya Y, Sasaki K, Ohmura K, Ishihama Y, Morinobu A, Iwai K. FEBS Lett. 2022 May;596(9):1147-1164. doi: 10.1002/1873-3468.14323. Epub 2022 Mar 4. PubMed
Mutation-specific reporter for optimization and enrichment of prime editing. Schene IF, Joore IP, Baijens JHL, Stevelink R, Kok G, Shehata S, Ilcken EF, Nieuwenhuis ECM, Bolhuis DP, van Rees RCM, Spelier SA, van der Doef HPJ, Beekman JM, Houwen RHJ, Nieuwenhuis EES, Fuchs SA. Nat Commun. 2022 Mar 1;13(1):1028. doi: 10.1038/s41467-022-28656-3. PubMed

Associated Plasmids
In vivo prime editing of a metabolic liver disease in mice. Bock D, Rothgangl T, Villiger L, Schmidheini L, Matsushita M, Mathis N, Ioannidi E, Rimann N, Grisch-Chan HM, Kreutzer S, Kontarakis Z, Kopf M, Thony B, Schwank G. Sci Transl Med. 2022 Mar 16;14(636):eabl9238. doi: 10.1126/scitranslmed.abl9238. Epub 2022 Mar 16. PubMed

Associated Plasmids
Reverting TP53 Mutation in Breast Cancer Cells: Prime Editing Workflow and Technical Considerations. Abuhamad AY, Mohamad Zamberi NN, Sheen L, Naes SM, Mohd Yusuf SNH, Ahmad Tajudin A, Mohtar MA, Amir Hamzah AS, Syafruddin SE. Cells. 2022 May 11;11(10). pii: cells11101612. doi: 10.3390/cells11101612. PubMed
Correction of Beta-Thalassemia IVS-II-654 Mutation in a Mouse Model Using Prime Editing. Zhang H, Sun R, Fei J, Chen H, Lu D. Int J Mol Sci. 2022 May 25;23(11). pii: ijms23115948. doi: 10.3390/ijms23115948. PubMed
Prime Editing Permits the Introduction of Specific Mutations in the Gene Responsible for Duchenne Muscular Dystrophy. Happi Mbakam C, Rousseau J, Tremblay G, Yameogo P, Tremblay JP. Int J Mol Sci. 2022 May 31;23(11). pii: ijms23116160. doi: 10.3390/ijms23116160. PubMed
Frequency and mechanisms of LINE-1 retrotransposon insertions at CRISPR/Cas9 sites. Tao J, Wang Q, Mendez-Dorantes C, Burns KH, Chiarle R. Nat Commun. 2022 Jun 27;13(1):3685. doi: 10.1038/s41467-022-31322-3. PubMed
A time-resolved, multi-symbol molecular recorder via sequential genome editing. Choi J, Chen W, Minkina A, Chardon FM, Suiter CC, Regalado SG, Domcke S, Hamazaki N, Lee C, Martin B, Daza RM, Shendure J. Nature. 2022 Jul 6. pii: 10.1038/s41586-022-04922-8. doi: 10.1038/s41586-022-04922-8. PubMed

Associated Plasmids
A truncated reverse transcriptase enhances prime editing by split AAV vectors. Gao Z, Ravendran S, Mikkelsen NS, Haldrup J, Cai H, Ding X, Paludan SR, Thomsen MK, Mikkelsen JG, Bak RO. Mol Ther. 2022 Jul 8. pii: S1525-0016(22)00422-1. doi: 10.1016/j.ymthe.2022.07.001. PubMed

Associated Plasmids
hnRNP R negatively regulates transcription by modulating the association of P-TEFb with 7SK and BRD4. Ji C, Deng C, Antor K, Bischler T, Schneider C, Fischer U, Sendtner M, Briese M. EMBO Rep. 2022 Sep 5;23(9):e55432. doi: 10.15252/embr.202255432. Epub 2022 Jul 20. PubMed
Designing and executing prime editing experiments in mammalian cells. Doman JL, Sousa AA, Randolph PB, Chen PJ, Liu DR. Nat Protoc. 2022 Nov;17(11):2431-2468. doi: 10.1038/s41596-022-00724-4. Epub 2022 Aug 8. PubMed
Targeted genomic translocations and inversions generated using a paired prime editing strategy. Kweon J, Hwang HY, Ryu H, Jang AH, Kim D, Kim Y. Mol Ther. 2023 Jan 4;31(1):249-259. doi: 10.1016/j.ymthe.2022.09.008. Epub 2022 Sep 15. PubMed
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites. Pihlajamaa P, Kauko O, Sahu B, Kivioja T, Taipale J. Nat Biotechnol. 2023 Feb;41(2):197-203. doi: 10.1038/s41587-022-01444-6. Epub 2022 Sep 26. PubMed
ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Chen C, Zhang Z, Liu C, Wang B, Liu P, Fang S, Yang F, You Y, Li X. Nat Commun. 2022 Oct 16;13(1):6108. doi: 10.1038/s41467-022-33859-9. PubMed
Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells. Li M, Zhong A, Wu Y, Sidharta M, Beaury M, Zhao X, Studer L, Zhou T. Nat Commun. 2022 Oct 27;13(1):6354. doi: 10.1038/s41467-022-34045-7. PubMed

Associated Plasmids
Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy. Nishiyama T, Zhang Y, Cui M, Li H, Sanchez-Ortiz E, McAnally JR, Tan W, Kim J, Chen K, Xu L, Bassel-Duby R, Olson EN. Sci Transl Med. 2022 Nov 23;14(672):eade1633. doi: 10.1126/scitranslmed.ade1633. Epub 2022 Nov 23. PubMed
Efficient and error-free correction of sickle mutation in human erythroid cells using prime editor-2. George A, Ravi NS, Prasad K, Panigrahi L, Koikkara S, Rajendiran V, Devaraju N, Paul J, Pai AA, Nakamura Y, Kurita R, Balasubramanian P, Thangavel S, Marepally S, Velayudhan SR, Srivastava A, Mohankumar KM. Front Genome Ed. 2022 Dec 20;4:1085111. doi: 10.3389/fgeed.2022.1085111. eCollection 2022. PubMed
Predicting prime editing efficiency and product purity by deep learning. Mathis N, Allam A, Kissling L, Marquart KF, Schmidheini L, Solari C, Balazs Z, Krauthammer M, Schwank G. Nat Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01613-7. PubMed

Associated Plasmids
Future Perspectives of Prime Editing for the Treatment of Inherited Retinal Diseases. Hansen S, McClements ME, Corydon TJ, MacLaren RE. Cells. 2023 Jan 29;12(3):440. doi: 10.3390/cells12030440. PubMed
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants. Koeppel J, Weller J, Peets EM, Pallaseni A, Kuzmin I, Raudvere U, Peterson H, Liberante FG, Parts L. Nat Biotechnol. 2023 Feb 16. doi: 10.1038/s41587-023-01678-y. PubMed
Prime editing with genuine Cas9 nickases minimizes unwanted indels. Lee J, Lim K, Kim A, Mok YG, Chung E, Cho SI, Lee JM, Kim JS. Nat Commun. 2023 Mar 30;14(1):1786. doi: 10.1038/s41467-023-37507-8. PubMed
An OMA1 redox site controls mitochondrial homeostasis, sarcoma growth, and immunogenicity. Miallot R, Millet V, Groult Y, Modelska A, Crescence L, Roulland S, Henri S, Malissen B, Brouilly N, Panicot-Dubois L, Vincentelli R, Sulzenbacher G, Finetti P, Dutour A, Blay JY, Bertucci F, Galland F, Naquet P. Life Sci Alliance. 2023 Apr 5;6(6):e202201767. doi: 10.26508/lsa.202201767. Print 2023 Jun. PubMed
Widespread RNA hypoediting in schizophrenia and its relevance to mitochondrial function. Choudhury M, Fu T, Amoah K, Jun HI, Chan TW, Park S, Walker DW, Bahn JH, Xiao X. Sci Adv. 2023 Apr 7;9(14):eade9997. doi: 10.1126/sciadv.ade9997. Epub 2023 Apr 7. PubMed
Prediction of efficiencies for diverse prime editing systems in multiple cell types. Yu G, Kim HK, Park J, Kwak H, Cheong Y, Kim D, Kim J, Kim J, Kim HH. Cell. 2023 Apr 21:S0092-8674(23)00331-8. doi: 10.1016/j.cell.2023.03.034. PubMed

Associated Plasmids
Generation of three CRISPR/Cas9 edited human induced pluripotent stem cell lines (DHMi005-A-5, DHMi005-A-6 and DHMi005-A-7) carrying a Holt-Oram Syndrome patient-specific TBX5 mutation with known cardiac phenotype and a FLAG-tag after exon 9 of the TBX5 gene. Lahm H, Stieglbauer S, Neb I, Doppler S, Schneider S, Dzilic E, Lange R, Krane M, Dressen M. Stem Cell Res. 2023 Jun;69:103123. doi: 10.1016/j.scr.2023.103123. Epub 2023 May 16. PubMed
Prime editing strategies to mediate exon skipping in DMD gene. Happi Mbakam C, Roustant J, Rousseau J, Yameogo P, Lu Y, Bigot A, Mamchaoui K, Mouly V, Lamothe G, Tremblay JP. Front Med (Lausanne). 2023 May 25;10:1128557. doi: 10.3389/fmed.2023.1128557. eCollection 2023. PubMed
Generation of sheep with defined FecB(B) and TBXT mutations and porcine blastocysts with KCNJ5(G151R/+) mutation using prime editing. Zhou S, Lenk LJ, Gao Y, Wang Y, Zhao X, Pan M, Huang S, Sun K, Kalds P, Luo Q, Lillico S, Sonstegard T, Scholl UI, Ma B, Petersen B, Chen Y, Wang X. BMC Genomics. 2023 Jun 12;24(1):313. doi: 10.1186/s12864-023-09409-y. PubMed
CRISPR prime editing for unconstrained correction of oncogenic KRAS variants. Jang G, Kweon J, Kim Y. Commun Biol. 2023 Jun 30;6(1):681. doi: 10.1038/s42003-023-05052-1. PubMed

Associated Plasmids
Chromatin structure and context-dependent sequence features control prime editing efficiency. Kim S, Yuan JB, Woods WS, Newton DA, Perez-Pinera P, Song JS. Front Genet. 2023 Jun 29;14:1222112. doi: 10.3389/fgene.2023.1222112. eCollection 2023. PubMed
A molecular proximity sensor based on an engineered, dual-component guide RNA. Choi J, Chen W, Liao H, Li X, Shendure J. bioRxiv [Preprint]. 2023 Aug 14:2023.08.14.553235. doi: 10.1101/2023.08.14.553235. PubMed
Genotoxic effects of base and prime editing in human hematopoietic stem cells. Fiumara M, Ferrari S, Omer-Javed A, Beretta S, Albano L, Canarutto D, Varesi A, Gaddoni C, Brombin C, Cugnata F, Zonari E, Naldini MM, Barcella M, Gentner B, Merelli I, Naldini L. Nat Biotechnol. 2023 Sep 7. doi: 10.1038/s41587-023-01915-4. PubMed
Generation of a tyrosine hydroxylase-2A-Cre knockin non-human primate model by homology-directed-repair-biased CRISPR genome editing. Yoshimatsu S, Okahara J, Yoshie J, Igarashi Y, Nakajima R, Sanosaka T, Qian E, Sato T, Kobayashi H, Morimoto S, Kishi N, Pillis DM, Malik P, Noce T, Okano H. Cell Rep Methods. 2023 Sep 25;3(9):100590. doi: 10.1016/j.crmeth.2023.100590. Epub 2023 Sep 14. PubMed
Flow cytometry-based quantification of genome editing efficiency in human cell lines using the L1CAM gene. Hasan MN, Hyodo T, Biswas M, Rahman ML, Mihara Y, Karnan S, Ota A, Tsuzuki S, Hosokawa Y, Konishi H. PLoS One. 2023 Nov 9;18(11):e0294146. doi: 10.1371/journal.pone.0294146. eCollection 2023. PubMed
Prime editing-mediated correction of the CFTR W1282X mutation in iPSCs and derived airway epithelial cells. Li C, Liu Z, Anderson J, Liu Z, Tang L, Li Y, Peng N, Chen J, Liu X, Fu L, Townes TM, Rowe SM, Bedwell DM, Guimbellot J, Zhao R. PLoS One. 2023 Nov 29;18(11):e0295009. doi: 10.1371/journal.pone.0295009. eCollection 2023. PubMed
iSCORE-PD: an isogenic stem cell collection to research Parkinson's Disease. Busquets O, Li H, Mohieddin Syed K, Jerez PA, Dunnack J, Bu RL, Verma Y, Pangilinan GR, Martin A, Straub J, Du Y, Simon VM, Poser S, Bush Z, Diaz J, Sahagun A, Gao J, Hernandez DG, Levine KS, Booth EO, Bateup HS, Rio DC, Hockemeyer D, Blauwendraat C, Soldner F. bioRxiv [Preprint]. 2024 Feb 13:2024.02.12.579917. doi: 10.1101/2024.02.12.579917. PubMed
Application of prime editing system to introduce TP53 R248Q hotspot mutation in acute lymphoblastic leukemia cell line. Nguyen T, Aida T, Iijima-Yamashita Y, Tamai M, Nagamachi A, Kagami K, Komatsu C, Kasai S, Akahane K, Goi K, Inaba T, Sanada M, Inukai T. Cancer Sci. 2024 Jun;115(6):1924-1935. doi: 10.1111/cas.16162. Epub 2024 Mar 28. PubMed
Improving prime editing with an endogenous small RNA-binding protein. Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y, Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC, Gilbert LA, Chan MM, Bauer DE, Marson A, Parsons LR, Adamson B. Nature. 2024 Apr 3. doi: 10.1038/s41586-024-07259-6. PubMed

Associated Plasmids
Massively parallel screen uncovers many rare 3' UTR variants regulating mRNA abundance of cancer driver genes. Fu T, Amoah K, Chan TW, Bahn JH, Lee JH, Terrazas S, Chong R, Kosuri S, Xiao X. Nat Commun. 2024 Apr 18;15(1):3335. doi: 10.1038/s41467-024-46795-7. PubMed
Precise editing of pathogenic nucleotide repeat expansions in iPSCs using paired prime editor. Hwang HY, Gim D, Yi H, Jung H, Lee J, Kim D. Nucleic Acids Res. 2024 Jun 10;52(10):5792-5803. doi: 10.1093/nar/gkae310. PubMed
Prime editing functionally corrects cystic fibrosis-causing CFTR mutations in human organoids and airway epithelial cells. Bulcaen M, Kortleven P, Liu RB, Maule G, Dreano E, Kelly M, Ensinck MM, Thierie S, Smits M, Ciciani M, Hatton A, Chevalier B, Ramalho AS, Casadevall I Solvas X, Debyser Z, Vermeulen F, Gijsbers R, Sermet-Gaudelus I, Cereseto A, Carlon MS. Cell Rep Med. 2024 May 21;5(5):101544. doi: 10.1016/j.xcrm.2024.101544. Epub 2024 May 1. PubMed

Associated Plasmids
Prime editing-mediated correction of the leptin receptor in muscle cells of db/db mice. Lee KE, Xu Y, Geng B, Kim J, Kellon N, He Z, Zhang Z, Li D, Gouchoe DA, Zhu H. Biotechnol J. 2024 May;19(5):e2300676. doi: 10.1002/biot.202300676. PubMed

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