pCMV-PE2 Citations (90)
Originally described in: Search-and-replace genome editing without double-strand breaks or donor DNA.Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. PubMed Journal
Articles Citing pCMV-PE2
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Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures.
Billon P, Nambiar TS, Hayward SB, Zafra MP, Schatoff EM, Oshima K, Dunbar A, Breinig M, Park YC, Ryu HS, Tschaharganeh DF, Levine RL, Baer R, Ferrando A, Dow LE, Ciccia A.
Cell Rep. 2020 Mar 10;30(10):3280-3295.e6. doi: 10.1016/j.celrep.2020.02.068.
PubMed
Associated Plasmids |
Highly Parallel Profiling of Cas9 Variant Specificity.
Schmid-Burgk JL, Gao L, Li D, Gardner Z, Strecker J, Lash B, Zhang F.
Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023.
PubMed
Associated Plasmids |
Predicting the efficiency of prime editing guide RNAs in human cells.
Kim HK, Yu G, Park J, Min S, Lee S, Yoon S, Kim HH.
Nat Biotechnol. 2020 Sep 21. pii: 10.1038/s41587-020-0677-y. doi: 10.1038/s41587-020-0677-y.
PubMed
Associated Plasmids |
| A web tool for the design of prime-editing guide RNAs. Chow RD, Chen JS, Shen J, Chen S. Nat Biomed Eng. 2021 Feb;5(2):190-194. doi: 10.1038/s41551-020-00622-8. Epub 2020 Sep 28. PubMed |
| Prime editing for functional repair in patient-derived disease models. Schene IF, Joore IP, Oka R, Mokry M, van Vugt AHM, van Boxtel R, van der Doef HPJ, van der Laan LJW, Verstegen MMA, van Hasselt PM, Nieuwenhuis EES, Fuchs SA. Nat Commun. 2020 Oct 23;11(1):5352. doi: 10.1038/s41467-020-19136-7. PubMed |
Precise genome engineering in Drosophila using prime editing.
Bosch JA, Birchak G, Perrimon N.
Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2021996118. doi: 10.1073/pnas.2021996118.
PubMed
Associated Plasmids |
| PrimeDesign software for rapid and simplified design of prime editing guide RNAs. Hsu JY, Grunewald J, Szalay R, Shih J, Anzalone AV, Lam KC, Shen MW, Petri K, Liu DR, Joung JK, Pinello L. Nat Commun. 2021 Feb 15;12(1):1034. doi: 10.1038/s41467-021-21337-7. PubMed |
Engineered Prime Editors with PAM flexibility.
Kweon J, Yoon JK, Jang AH, Shin HR, See JE, Jang G, Kim JI, Kim Y.
Mol Ther. 2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 10.1016/j.ymthe.2021.02.022.
PubMed
Associated Plasmids |
| Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression. Gao P, Lyu Q, Ghanam AR, Lazzarotto CR, Newby GA, Zhang W, Choi M, Slivano OJ, Holden K, Walker JA 2nd, Kadina AP, Munroe RJ, Abratte CM, Schimenti JC, Liu DR, Tsai SQ, Long X, Miano JM. Genome Biol. 2021 Mar 16;22(1):83. doi: 10.1186/s13059-021-02304-3. PubMed |
| Efficient correction of Duchenne muscular dystrophy mutations by SpCas9 and dual gRNAs. Xiang X, Zhao X, Pan X, Dong Z, Yu J, Li S, Liang X, Han P, Qu K, Jensen JB, Farup J, Wang F, Petersen TS, Bolund L, Teng H, Lin L, Luo Y. Mol Ther Nucleic Acids. 2021 Mar 13;24:403-415. doi: 10.1016/j.omtn.2021.03.005. eCollection 2021 Jun 4. PubMed |
CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells.
Petri K, Zhang W, Ma J, Schmidts A, Lee H, Horng JE, Kim DY, Kurt IC, Clement K, Hsu JY, Pinello L, Maus MV, Joung JK, Yeh JJ.
Nat Biotechnol. 2021 Apr 29. pii: 10.1038/s41587-021-00901-y. doi: 10.1038/s41587-021-00901-y.
PubMed
Associated Plasmids |
| Adenine base editing and prime editing of chemically derived hepatic progenitors rescue genetic liver disease. Kim Y, Hong SA, Yu J, Eom J, Jang K, Yoon S, Hong DH, Seo D, Lee SN, Woo JS, Jeong J, Bae S, Choi D. Cell Stem Cell. 2021 Sep 2;28(9):1614-1624.e5. doi: 10.1016/j.stem.2021.04.010. Epub 2021 May 4. PubMed |
Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor.
Park SJ, Jeong TY, Shin SK, Yoon DE, Lim SY, Kim SP, Choi J, Lee H, Hong JI, Ahn J, Seong JK, Kim K.
Genome Biol. 2021 Jun 3;22(1):170. doi: 10.1186/s13059-021-02389-w.
PubMed
Associated Plasmids |
| Dual-AAV delivering split prime editor system for in vivo genome editing. Zhi S, Chen Y, Wu G, Wen J, Wu J, Liu Q, Li Y, Kang R, Hu S, Wang J, Liang P, Huang J. Mol Ther. 2021 Jul 21. pii: S1525-0016(21)00365-8. doi: 10.1016/j.ymthe.2021.07.011. PubMed |
Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases.
Jang H, Jo DH, Cho CS, Shin JH, Seo JH, Yu G, Gopalappa R, Kim D, Cho SR, Kim JH, Kim HH.
Nat Biomed Eng. 2021 Aug 26. pii: 10.1038/s41551-021-00788-9. doi: 10.1038/s41551-021-00788-9.
PubMed
Associated Plasmids |
Optimized nickase- and nuclease-based prime editing in human and mouse cells.
Adikusuma F, Lushington C, Arudkumar J, Godahewa GI, Chey YCJ, Gierus L, Piltz S, Geiger A, Jain Y, Reti D, Wilson LOW, Bauer DC, Thomas PQ.
Nucleic Acids Res. 2021 Sep 17. pii: 6371974. doi: 10.1093/nar/gkab792.
PubMed
Associated Plasmids |
| Modeling a cataract disorder in mice with prime editing. Lin J, Liu X, Lu Z, Huang S, Wu S, Yu W, Liu Y, Zheng X, Huang X, Sun Q, Qiao Y, Liu Z. Mol Ther Nucleic Acids. 2021 Jul 2;25:494-501. doi: 10.1016/j.omtn.2021.06.020. eCollection 2021 Sep 3. PubMed |
Precise genomic deletions using paired prime editing.
Choi J, Chen W, Suiter CC, Lee C, Chardon FM, Yang W, Leith A, Daza RM, Martin B, Shendure J.
Nat Biotechnol. 2021 Oct 14. pii: 10.1038/s41587-021-01025-z. doi: 10.1038/s41587-021-01025-z.
PubMed
Associated Plasmids |
Enhanced prime editing systems by manipulating cellular determinants of editing outcomes.
Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, Liu DR.
Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018.
PubMed
Associated Plasmids |
| Modelling of primary ciliary dyskinesia using patient-derived airway organoids. van der Vaart J, Bottinger L, Geurts MH, van de Wetering WJ, Knoops K, Sachs N, Begthel H, Korving J, Lopez-Iglesias C, Peters PJ, Eitan K, Gileles-Hillel A, Clevers H. EMBO Rep. 2021 Dec 6;22(12):e52058. doi: 10.15252/embr.202052058. Epub 2021 Oct 25. PubMed |
| Delete_A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines. Eggenschwiler R, Gschwendtberger T, Felski C, Jahn C, Langer F, Sterneckert J, Hermann A, Luhmann J, Steinemann D, Haase A, Martin U, Petri S, Cantz T. Sci Rep. 2021 Nov 12;11(1):22154. doi: 10.1038/s41598-021-01689-2. PubMed |
piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition.
Wolff JH, Haldrup J, Thomsen EA, Andersen S, Mikkelsen JG.
Front Genome Ed. 2021 Nov 12;3:786893. doi: 10.3389/fgeed.2021.786893. eCollection 2021.
PubMed
Associated Plasmids |
| Random-PE: an efficient integration of random sequences into mammalian genome by prime editing. Jiao Y, Zhou L, Tao R, Wang Y, Hu Y, Jiang L, Li L, Yao S. Mol Biomed. 2021 Nov 18;2(1):36. doi: 10.1186/s43556-021-00057-w. PubMed |
| Comprehensive analysis of prime editing outcomes in human embryonic stem cells. Habib O, Habib G, Hwang GH, Bae S. Nucleic Acids Res. 2022 Jan 25;50(2):1187-1197. doi: 10.1093/nar/gkab1295. PubMed |
| Insertion of the Icelandic Mutation (A673T) by Prime Editing: A Potential Preventive Treatment for Familial and Sporadic Alzheimer's Disease. Tremblay G, Rousseau J, Mbakam CH, Tremblay JP. CRISPR J. 2022 Feb;5(1):109-122. doi: 10.1089/crispr.2021.0085. Epub 2022 Feb 7. PubMed |
| Discovery of potent and versatile CRISPR-Cas9 inhibitors engineered for chemically controllable genome editing. Song G, Zhang F, Tian C, Gao X, Zhu X, Fan D, Tian Y. Nucleic Acids Res. 2022 Mar 21;50(5):2836-2853. doi: 10.1093/nar/gkac099. PubMed |
| Saturation variant interpretation using CRISPR prime editing. Erwood S, Bily TMI, Lequyer J, Yan J, Gulati N, Brewer RA, Zhou L, Pelletier L, Ivakine EA, Cohn RD. Nat Biotechnol. 2022 Jun;40(6):885-895. doi: 10.1038/s41587-021-01201-1. Epub 2022 Feb 21. PubMed |
| ABIN1 is a signal-induced autophagy receptor that attenuates NF-kappaB activation by recognizing linear ubiquitin chains. Shinkawa Y, Imami K, Fuseya Y, Sasaki K, Ohmura K, Ishihama Y, Morinobu A, Iwai K. FEBS Lett. 2022 May;596(9):1147-1164. doi: 10.1002/1873-3468.14323. Epub 2022 Mar 4. PubMed |
Mutation-specific reporter for optimization and enrichment of prime editing.
Schene IF, Joore IP, Baijens JHL, Stevelink R, Kok G, Shehata S, Ilcken EF, Nieuwenhuis ECM, Bolhuis DP, van Rees RCM, Spelier SA, van der Doef HPJ, Beekman JM, Houwen RHJ, Nieuwenhuis EES, Fuchs SA.
Nat Commun. 2022 Mar 1;13(1):1028. doi: 10.1038/s41467-022-28656-3.
PubMed
Associated Plasmids |
In vivo prime editing of a metabolic liver disease in mice.
Bock D, Rothgangl T, Villiger L, Schmidheini L, Matsushita M, Mathis N, Ioannidi E, Rimann N, Grisch-Chan HM, Kreutzer S, Kontarakis Z, Kopf M, Thony B, Schwank G.
Sci Transl Med. 2022 Mar 16;14(636):eabl9238. doi: 10.1126/scitranslmed.abl9238. Epub 2022 Mar 16.
PubMed
Associated Plasmids |
| Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing. Peterka M, Akrap N, Li S, Wimberger S, Hsieh PP, Degtev D, Bestas B, Barr J, van de Plassche S, Mendoza-Garcia P, Svikovic S, Sienski G, Firth M, Maresca M. Nat Commun. 2022 Mar 24;13(1):1240. doi: 10.1038/s41467-022-28771-1. PubMed |
| Development and Optimization of CRISPR Prime Editing System in Photoautotrophic Cells. Jiang Z, Abdullah, Zhang S, Jiang Y, Liu R, Xiao Y. Molecules. 2022 Mar 8;27(6). pii: molecules27061758. doi: 10.3390/molecules27061758. PubMed |
| Enhancement of prime editing via xrRNA motif-joined pegRNA. Zhang G, Liu Y, Huang S, Qu S, Cheng D, Yao Y, Ji Q, Wang X, Huang X, Liu J. Nat Commun. 2022 Apr 6;13(1):1856. doi: 10.1038/s41467-022-29507-x. PubMed |
| Expansion of the prime editing modality with Cas9 from Francisella novicida. Oh Y, Lee WJ, Hur JK, Song WJ, Lee Y, Kim H, Gwon LW, Kim YH, Park YH, Kim CH, Lim KS, Song BS, Huh JW, Kim SU, Jun BH, Jung C, Lee SH. Genome Biol. 2022 Apr 11;23(1):92. doi: 10.1186/s13059-022-02644-8. PubMed |
| WT-PE: Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing. Tao R, Wang Y, Hu Y, Jiao Y, Zhou L, Jiang L, Li L, He X, Li M, Yu Y, Chen Q, Yao S. Signal Transduct Target Ther. 2022 Apr 20;7(1):108. doi: 10.1038/s41392-022-00936-w. PubMed |
| Targeting double-strand break indel byproducts with secondary guide RNAs improves Cas9 HDR-mediated genome editing efficiencies. Bodai Z, Bishop AL, Gantz VM, Komor AC. Nat Commun. 2022 May 9;13(1):2351. doi: 10.1038/s41467-022-29989-9. PubMed |
Multiplex base- and prime-editing with drive-and-process CRISPR arrays.
Yuan Q, Gao X.
Nat Commun. 2022 May 19;13(1):2771. doi: 10.1038/s41467-022-30514-1.
PubMed
Associated Plasmids |
| Reverting TP53 Mutation in Breast Cancer Cells: Prime Editing Workflow and Technical Considerations. Abuhamad AY, Mohamad Zamberi NN, Sheen L, Naes SM, Mohd Yusuf SNH, Ahmad Tajudin A, Mohtar MA, Amir Hamzah AS, Syafruddin SE. Cells. 2022 May 11;11(10). pii: cells11101612. doi: 10.3390/cells11101612. PubMed |
| Correction of Beta-Thalassemia IVS-II-654 Mutation in a Mouse Model Using Prime Editing. Zhang H, Sun R, Fei J, Chen H, Lu D. Int J Mol Sci. 2022 May 25;23(11). pii: ijms23115948. doi: 10.3390/ijms23115948. PubMed |
| Prime Editing Permits the Introduction of Specific Mutations in the Gene Responsible for Duchenne Muscular Dystrophy. Happi Mbakam C, Rousseau J, Tremblay G, Yameogo P, Tremblay JP. Int J Mol Sci. 2022 May 31;23(11). pii: ijms23116160. doi: 10.3390/ijms23116160. PubMed |
| Bi-PE: bi-directional priming improves CRISPR/Cas9 prime editing in mammalian cells. Tao R, Wang Y, Jiao Y, Hu Y, Li L, Jiang L, Zhou L, Qu J, Chen Q, Yao S. Nucleic Acids Res. 2022 Jun 24;50(11):6423-6434. doi: 10.1093/nar/gkac506. PubMed |
| Therapeutic base editing and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosa. Hong SA, Kim SE, Lee AY, Hwang GH, Kim JH, Iwata H, Kim SC, Bae S, Lee SE. Mol Ther. 2022 Aug 3;30(8):2664-2679. doi: 10.1016/j.ymthe.2022.06.005. Epub 2022 Jun 10. PubMed |
| Frequency and mechanisms of LINE-1 retrotransposon insertions at CRISPR/Cas9 sites. Tao J, Wang Q, Mendez-Dorantes C, Burns KH, Chiarle R. Nat Commun. 2022 Jun 27;13(1):3685. doi: 10.1038/s41467-022-31322-3. PubMed |
| HDAC inhibitors improve CRISPR-Cas9 mediated prime editing and base editing. Liu N, Zhou L, Lin G, Hu Y, Jiao Y, Wang Y, Liu J, Yang S, Yao S. Mol Ther Nucleic Acids. 2022 Jun 2;29:36-46. doi: 10.1016/j.omtn.2022.05.036. eCollection 2022 Sep 13. PubMed |
| Broadening prime editing toolkits using RNA-Pol-II-driven engineered pegRNA. Huang S, Zhang Z, Tao W, Liu Y, Li X, Wang X, Harati J, Wang PY, Huang X, Lin CP. Mol Ther. 2022 Sep 7;30(9):2923-2932. doi: 10.1016/j.ymthe.2022.07.002. Epub 2022 Jul 6. PubMed |
A truncated reverse transcriptase enhances prime editing by split AAV vectors.
Gao Z, Ravendran S, Mikkelsen NS, Haldrup J, Cai H, Ding X, Paludan SR, Thomsen MK, Mikkelsen JG, Bak RO.
Mol Ther. 2022 Jul 8. pii: S1525-0016(22)00422-1. doi: 10.1016/j.ymthe.2022.07.001.
PubMed
Associated Plasmids |
| hnRNP R negatively regulates transcription by modulating the association of P-TEFb with 7SK and BRD4. Ji C, Deng C, Antor K, Bischler T, Schneider C, Fischer U, Sendtner M, Briese M. EMBO Rep. 2022 Sep 5;23(9):e55432. doi: 10.15252/embr.202255432. Epub 2022 Jul 20. PubMed |
| Targeted-Deletion of a Tiny Sequence via Prime Editing to Restore SMN Expression. Zhou M, Tang S, Duan N, Xie M, Li Z, Feng M, Wu L, Hu Z, Liang D. Int J Mol Sci. 2022 Jul 19;23(14):7941. doi: 10.3390/ijms23147941. PubMed |
| Targeted genomic translocations and inversions generated using a paired prime editing strategy. Kweon J, Hwang HY, Ryu H, Jang AH, Kim D, Kim Y. Mol Ther. 2023 Jan 4;31(1):249-259. doi: 10.1016/j.ymthe.2022.09.008. Epub 2022 Sep 15. PubMed |
| A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites. Pihlajamaa P, Kauko O, Sahu B, Kivioja T, Taipale J. Nat Biotechnol. 2023 Feb;41(2):197-203. doi: 10.1038/s41587-022-01444-6. Epub 2022 Sep 26. PubMed |
Precise DNA cleavage using CRISPR-SpRYgests.
Christie KA, Guo JA, Silverstein RA, Doll RM, Mabuchi M, Stutzman HE, Lin J, Ma L, Walton RT, Pinello L, Robb GB, Kleinstiver BP.
Nat Biotechnol. 2022 Oct 6. pii: 10.1038/s41587-022-01492-y. doi: 10.1038/s41587-022-01492-y.
PubMed
Associated Plasmids |
Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells.
Li M, Zhong A, Wu Y, Sidharta M, Beaury M, Zhao X, Studer L, Zhou T.
Nat Commun. 2022 Oct 27;13(1):6354. doi: 10.1038/s41467-022-34045-7.
PubMed
Associated Plasmids |
| Challenges in Gene Therapy for Somatic Reverted Mosaicism in X-Linked Combined Immunodeficiency by CRISPR/Cas9 and Prime Editing. Hou Y, Urena-Bailen G, Mohammadian Gol T, Gratz PG, Gratz HP, Roig-Merino A, Antony JS, Lamsfus-Calle A, Daniel-Moreno A, Handgretinger R, Mezger M. Genes (Basel). 2022 Dec 13;13(12):2348. doi: 10.3390/genes13122348. PubMed |
| Efficient and error-free correction of sickle mutation in human erythroid cells using prime editor-2. George A, Ravi NS, Prasad K, Panigrahi L, Koikkara S, Rajendiran V, Devaraju N, Paul J, Pai AA, Nakamura Y, Kurita R, Balasubramanian P, Thangavel S, Marepally S, Velayudhan SR, Srivastava A, Mohankumar KM. Front Genome Ed. 2022 Dec 20;4:1085111. doi: 10.3389/fgeed.2022.1085111. eCollection 2022. PubMed |
Engineered PROTAC-CID Systems for Mammalian Inducible Gene Regulation.
Ma D, Yuan Q, Peng F, Paredes V, Zeng H, Osikpa EC, Yang Q, Peddi A, Patel A, Liu MS, Sun Z, Gao X.
J Am Chem Soc. 2023 Jan 25;145(3):1593-1606. doi: 10.1021/jacs.2c09129. Epub 2023 Jan 10.
PubMed
Associated Plasmids |
Predicting prime editing efficiency and product purity by deep learning.
Mathis N, Allam A, Kissling L, Marquart KF, Schmidheini L, Solari C, Balazs Z, Krauthammer M, Schwank G.
Nat Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01613-7.
PubMed
Associated Plasmids |
| Dual-AAV split prime editor corrects the mutation and phenotype in mice with inherited retinal degeneration. She K, Liu Y, Zhao Q, Jin X, Yang Y, Su J, Li R, Song L, Xiao J, Yao S, Lu F, Wei Y, Yang Y. Signal Transduct Target Ther. 2023 Feb 6;8(1):57. doi: 10.1038/s41392-022-01234-1. PubMed |
| Engineered human hepatocyte organoids enable CRISPR-based target discovery and drug screening for steatosis. Hendriks D, Brouwers JF, Hamer K, Geurts MH, Luciana L, Massalini S, Lopez-Iglesias C, Peters PJ, Rodriguez-Colman MJ, Chuva de Sousa Lopes S, Artegiani B, Clevers H. Nat Biotechnol. 2023 Feb 23. doi: 10.1038/s41587-023-01680-4. PubMed |
| A chemically controlled Cas9 switch enables temporal modulation of diverse effectors. Wei CT, Popp NA, Peleg O, Powell RL, Borenstein E, Maly DJ, Fowler DM. Nat Chem Biol. 2023 Aug;19(8):981-991. doi: 10.1038/s41589-023-01278-6. Epub 2023 Mar 6. PubMed |
| Prime editing with genuine Cas9 nickases minimizes unwanted indels. Lee J, Lim K, Kim A, Mok YG, Chung E, Cho SI, Lee JM, Kim JS. Nat Commun. 2023 Mar 30;14(1):1786. doi: 10.1038/s41467-023-37507-8. PubMed |
| An OMA1 redox site controls mitochondrial homeostasis, sarcoma growth, and immunogenicity. Miallot R, Millet V, Groult Y, Modelska A, Crescence L, Roulland S, Henri S, Malissen B, Brouilly N, Panicot-Dubois L, Vincentelli R, Sulzenbacher G, Finetti P, Dutour A, Blay JY, Bertucci F, Galland F, Naquet P. Life Sci Alliance. 2023 Apr 5;6(6):e202201767. doi: 10.26508/lsa.202201767. Print 2023 Jun. PubMed |
| Widespread RNA hypoediting in schizophrenia and its relevance to mitochondrial function. Choudhury M, Fu T, Amoah K, Jun HI, Chan TW, Park S, Walker DW, Bahn JH, Xiao X. Sci Adv. 2023 Apr 7;9(14):eade9997. doi: 10.1126/sciadv.ade9997. Epub 2023 Apr 7. PubMed |
| Quantitative proteomics and phosphoproteomics of PPP2R5D variants reveal deregulation of RPS6 phosphorylation through converging signaling cascades. Ka S, Cm P, Swingle MR, A M, C L, Ad C, Re H, An K. bioRxiv. 2023 Mar 27:2023.03.27.534397. doi: 10.1101/2023.03.27.534397. Preprint. PubMed |
A prime editor mouse to model a broad spectrum of somatic mutations in vivo.
Ely ZA, Mathey-Andrews N, Naranjo S, Gould SI, Mercer KL, Newby GA, Cabana CM, Rideout WM 3rd, Jaramillo GC, Khirallah JM, Holland K, Randolph PB, Freed-Pastor WA, Davis JR, Kulstad Z, Westcott PMK, Lin L, Anzalone AV, Horton BL, Pattada NB, Shanahan SL, Ye Z, Spranger S, Xu Q, Sanchez-Rivera FJ, Liu DR, Jacks T.
Nat Biotechnol. 2023 May 11. doi: 10.1038/s41587-023-01783-y.
PubMed
Associated Plasmids |
| Generation of three CRISPR/Cas9 edited human induced pluripotent stem cell lines (DHMi005-A-5, DHMi005-A-6 and DHMi005-A-7) carrying a Holt-Oram Syndrome patient-specific TBX5 mutation with known cardiac phenotype and a FLAG-tag after exon 9 of the TBX5 gene. Lahm H, Stieglbauer S, Neb I, Doppler S, Schneider S, Dzilic E, Lange R, Krane M, Dressen M. Stem Cell Res. 2023 Jun;69:103123. doi: 10.1016/j.scr.2023.103123. Epub 2023 May 16. PubMed |
| Targeted, programmable, and precise tandem duplication in the mammalian genome. Jiao Y, Li M, He X, Wang Y, Song J, Hu Y, Li L, Zhou L, Jiang L, Qu J, Xie L, Chen Q, Yao S. Genome Res. 2023 May;33(5):779-786. doi: 10.1101/gr.277261.122. Epub 2023 Jun 9. PubMed |
| Prime editing strategies to mediate exon skipping in DMD gene. Happi Mbakam C, Roustant J, Rousseau J, Yameogo P, Lu Y, Bigot A, Mamchaoui K, Mouly V, Lamothe G, Tremblay JP. Front Med (Lausanne). 2023 May 25;10:1128557. doi: 10.3389/fmed.2023.1128557. eCollection 2023. PubMed |
| Generation of sheep with defined FecB(B) and TBXT mutations and porcine blastocysts with KCNJ5(G151R/+) mutation using prime editing. Zhou S, Lenk LJ, Gao Y, Wang Y, Zhao X, Pan M, Huang S, Sun K, Kalds P, Luo Q, Lillico S, Sonstegard T, Scholl UI, Ma B, Petersen B, Chen Y, Wang X. BMC Genomics. 2023 Jun 12;24(1):313. doi: 10.1186/s12864-023-09409-y. PubMed |
| High-efficiency targeted transgene integration via primed micro-homologues. Wang C, Fang S, Chen Y, Tang N, Jiao G, Hu Y, Li J, Shan Q, Wang X, Feng G, Zhou Q, Li W. Cell Discov. 2023 Jul 4;9(1):69. doi: 10.1038/s41421-023-00552-0. PubMed |
| Generation of an Avian Myeloblastosis Virus (AMV) Reverse Transcriptase Prime Editor. Tsai YT, da Costa BL, Caruso SM, Nolan ND, Levi SR, Tsang SH, Quinn PMJ. Adv Exp Med Biol. 2023;1415:109-114. doi: 10.1007/978-3-031-27681-1_17. PubMed |
| Chromatin structure and context-dependent sequence features control prime editing efficiency. Kim S, Yuan JB, Woods WS, Newton DA, Perez-Pinera P, Song JS. Front Genet. 2023 Jun 29;14:1222112. doi: 10.3389/fgene.2023.1222112. eCollection 2023. PubMed |
| APOE loss-of-function variants: Compatible with longevity and associated with resistance to Alzheimer's Disease pathology. Chemparathy A, Guen YL, Chen S, Lee EG, Leong L, Gorzynski J, Xu G, Belloy M, Kasireddy N, Tauber AP, Williams K, Stewart I, Wingo T, Lah J, Jayadev S, Hales C, Peskind E, Child DD, Keene CD, Cong L, Ashley E, Yu CE, Greicius MD. medRxiv [Preprint]. 2023 Jul 24:2023.07.20.23292771. doi: 10.1101/2023.07.20.23292771. PubMed |
| Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription. Xie J, Zhuang Z, Gou S, Zhang Q, Wang X, Lan T, Lian M, Li N, Liang Y, Ouyang Z, Ye Y, Wu H, Lai L, Wang K. Nucleic Acids Res. 2023 Oct 13;51(18):10075-10093. doi: 10.1093/nar/gkad687. PubMed |
| Genotoxic effects of base and prime editing in human hematopoietic stem cells. Fiumara M, Ferrari S, Omer-Javed A, Beretta S, Albano L, Canarutto D, Varesi A, Gaddoni C, Brombin C, Cugnata F, Zonari E, Naldini MM, Barcella M, Gentner B, Merelli I, Naldini L. Nat Biotechnol. 2023 Sep 7. doi: 10.1038/s41587-023-01915-4. PubMed |
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| Generation of a tyrosine hydroxylase-2A-Cre knockin non-human primate model by homology-directed-repair-biased CRISPR genome editing. Yoshimatsu S, Okahara J, Yoshie J, Igarashi Y, Nakajima R, Sanosaka T, Qian E, Sato T, Kobayashi H, Morimoto S, Kishi N, Pillis DM, Malik P, Noce T, Okano H. Cell Rep Methods. 2023 Sep 25;3(9):100590. doi: 10.1016/j.crmeth.2023.100590. Epub 2023 Sep 14. PubMed |
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| Prime editing-mediated correction of the CFTR W1282X mutation in iPSCs and derived airway epithelial cells. Li C, Liu Z, Anderson J, Liu Z, Tang L, Li Y, Peng N, Chen J, Liu X, Fu L, Townes TM, Rowe SM, Bedwell DM, Guimbellot J, Zhao R. PLoS One. 2023 Nov 29;18(11):e0295009. doi: 10.1371/journal.pone.0295009. eCollection 2023. PubMed |
| Rapid modeling of an ultra-rare epilepsy variant in wild-type mice by in utero prime editing. Robertson CD, Davis P, Richardson RR, Iffland PH 2nd, Vieira DCO, Steyert M, McKeon PN, Romanowski AJ, Crutcher G, Jasarevic E, Wolff SBE, Mathur BN, Crino PB, Bale TL, Dick IE, Poulopoulos A. bioRxiv [Preprint]. 2023 Dec 19:2023.12.06.570164. doi: 10.1101/2023.12.06.570164. PubMed |
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| Successful Correction by Prime Editing of a Mutation in the RYR1 Gene Responsible for a Myopathy. Godbout K, Rousseau J, Tremblay JP. Cells. 2023 Dec 22;13(1):31. doi: 10.3390/cells13010031. PubMed |
| Enhancing prime editor activity by directed protein evolution in yeast. Weber Y, Bock D, Ivascu A, Mathis N, Rothgangl T, Ioannidi EI, Blaudt AC, Tidecks L, Vadovics M, Muramatsu H, Reichmuth A, Marquart KF, Kissling L, Pardi N, Jinek M, Schwank G. Nat Commun. 2024 Mar 7;15(1):2092. doi: 10.1038/s41467-024-46107-z. PubMed |
| Application of prime editing system to introduce TP53 R248Q hotspot mutation in acute lymphoblastic leukemia cell line. Nguyen T, Aida T, Iijima-Yamashita Y, Tamai M, Nagamachi A, Kagami K, Komatsu C, Kasai S, Akahane K, Goi K, Inaba T, Sanada M, Inukai T. Cancer Sci. 2024 Jun;115(6):1924-1935. doi: 10.1111/cas.16162. Epub 2024 Mar 28. PubMed |
Improving prime editing with an endogenous small RNA-binding protein.
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| Enhancing prime editor flexibility with coiled-coil heterodimers. Mu S, Chen H, Li Q, Gou S, Liu X, Wang J, Zheng W, Chen M, Jin Q, Lai L, Wang K, Shi H. Genome Biol. 2024 Apr 26;25(1):108. doi: 10.1186/s13059-024-03257-z. PubMed |
Prime editing functionally corrects cystic fibrosis-causing CFTR mutations in human organoids and airway epithelial cells.
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