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Addgene

pIM27
(Plasmid #132585)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132585 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZA4
  • Backbone size w/o insert (bp) 2055
  • Total vector size (bp) 4050
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    oxySp-mCherry-TEVrs-LAA-TermT1
  • Insert Size (bp)
    1046
  • Promoter oxySp
  • Tag / Fusion Protein
    • TEVrs-LAA (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCGAACAGGCTTATGTCCAC
  • 3′ sequencing primer GGTCAGTGCGTCCTGCTGATGTTG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    pLsoxS-mCherry-TermT1
  • Insert Size (bp)
    949
  • Promoter pLsoxS

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTCGAGCCATAAATCGCTTTACCTCAAGTT
  • 3′ sequencing primer TGGCATCTTCCAGGAAATCT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIM27 was a gift from Timothy Lu (Addgene plasmid # 132585 ; http://n2t.net/addgene:132585 ; RRID:Addgene_132585)

  • For your References section:

    Gene networks that compensate for crosstalk with crosstalk. Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK. Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y. 10.1038/s41467-019-12021-y PubMed 31492904
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