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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13250 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRV.GFP
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFOXP3
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SpeciesH. sapiens (human)
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Entrez GeneFOXP3 (a.k.a. AIID, DIETER, IPEX, JM2, PIDX, XPID)
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer IRES-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pRV GFP has IRES, EGFP, MMLV LTR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRV.GFP FOXP3 was a gift from Anjana Rao (Addgene plasmid # 13250 ; http://n2t.net/addgene:13250 ; RRID:Addgene_13250) -
For your References section:
FOXP3 controls regulatory T cell function through cooperation with NFAT. Wu Y, Borde M, Heissmeyer V, Feuerer M, Lapan AD, Stroud JC, Bates DL, Guo L, Han A, Ziegler SF, Mathis D, Benoist C, Chen L, Rao A. Cell. 2006 Jul 28. 126(2):375-87. 10.1016/j.cell.2006.05.042 PubMed 16873067