pcDNA3.1-Flag-NPM1wt
(Plasmid
#131810)
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PurposeExpresses Flag-NPM1wildtype (human) in mammalian cells through transient transfection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131810 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNPM1
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SpeciesH. sapiens (human)
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Entrez GeneNPM1 (a.k.a. B23, NPM)
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr Charles Sherr
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-Flag-NPM1wt was a gift from Mark Minden (Addgene plasmid # 131810 ; http://n2t.net/addgene:131810 ; RRID:Addgene_131810) -
For your References section:
NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia. Wang AJ, Han Y, Jia N, Chen P, Minden MD. Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12. 10.1038/s41375-019-0681-8 PubMed 31831844