pcDNA3.1(+)- e18
(Plasmid #131670)

• Purpose: Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.
• Depositing Lab: Alberto Ciccia
• Publication: Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi: 10.1038/s41467-019-11105-z.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 5444
• Total vector size (bp): 6821
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: e18 (engineered RAD18 with SAP domain deleted)
• Species: H. sapiens (human)
• Insert Size (bp): 1377
• GenBank ID:
• Entrez Gene: RAD18 (a.k.a. RNF73)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on backbone)
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • Annotated Sequence
• A portion of this plasmid was derived from a plasmid made by: RAD18 gene was obtained from the ORFeome v7 (ID:1782)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry