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Addgene

pLOC-MCOLN1
(Plasmid #131583)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131583 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLOC
  • Backbone manufacturer
    Dharmacon
  • Backbone size w/o insert (bp) 9089
  • Total vector size (bp) 10832
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCOLN1
  • Alt name
    TRPML1, Mucolipin-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1743
  • Entrez Gene
    MCOLN1 (a.k.a. LECD, MG-2, ML1, ML4, MLIV, MST080, MSTP080, TRP-ML1, TRPM-L1, TRPML1)
  • Promoter CMV
  • Tag / Fusion Protein
    • turboGFP tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The ORF was from DNASU

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLOC-MCOLN1 was a gift from Sean Morrison (Addgene plasmid # 131583 ; http://n2t.net/addgene:131583 ; RRID:Addgene_131583)
  • For your References section:

    TRPML1 Promotes Protein Homeostasis in Melanoma Cells by Negatively Regulating MAPK and mTORC1 Signaling. Kasitinon SY, Eskiocak U, Martin M, Bezwada D, Khivansara V, Tasdogan A, Zhao Z, Mathews T, Aurora AB, Morrison SJ. Cell Rep. 2019 Aug 27;28(9):2293-2305.e9. doi: 10.1016/j.celrep.2019.07.086. 10.1016/j.celrep.2019.07.086 PubMed 31461647