pLOC-MCOLN1
(Plasmid
#131583)
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PurposeFor expression of human MCOLN1 in mammalian cells, GFP-tagged
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLOC
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Backbone manufacturerDharmacon
- Backbone size w/o insert (bp) 9089
- Total vector size (bp) 10832
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCOLN1
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Alt nameTRPML1, Mucolipin-1
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SpeciesH. sapiens (human)
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Insert Size (bp)1743
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Entrez GeneMCOLN1 (a.k.a. LECD, MG-2, ML1, ML4, MLIV, MST080, MSTP080, TRP-ML1, TRPM-L1, TRPML1)
- Promoter CMV
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Tag
/ Fusion Protein
- turboGFP tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe ORF was from DNASU
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLOC-MCOLN1 was a gift from Sean Morrison (Addgene plasmid # 131583 ; http://n2t.net/addgene:131583 ; RRID:Addgene_131583) -
For your References section:
TRPML1 Promotes Protein Homeostasis in Melanoma Cells by Negatively Regulating MAPK and mTORC1 Signaling. Kasitinon SY, Eskiocak U, Martin M, Bezwada D, Khivansara V, Tasdogan A, Zhao Z, Mathews T, Aurora AB, Morrison SJ. Cell Rep. 2019 Aug 27;28(9):2293-2305.e9. doi: 10.1016/j.celrep.2019.07.086. 10.1016/j.celrep.2019.07.086 PubMed 31461647