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Addgene

pcDNA-Flag-Trib3
(Plasmid #131157)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131157 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Trib3
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Trib3 (a.k.a. Ifld2, Nipk, SINK, SKIP3, TRB-3, Trb3)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Murine Trib3 with an N-terminal Flag tag cloned into BamHI-EcoRI site (with Bgl2 so BamHI site is destroyed). Release insert with HindIII and XhoI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-Flag-Trib3 was a gift from Karen Keeshan & Warren Pear (Addgene plasmid # 131157 ; http://n2t.net/addgene:131157 ; RRID:Addgene_131157)

  • For your References section:

    Differential ability of Tribbles family members to promote degradation of C/EBPalpha and induce acute myelogenous leukemia. Dedhia PH, Keeshan K, Uljon S, Xu L, Vega ME, Shestova O, Zaks-Zilberman M, Romany C, Blacklow SC, Pear WS. Blood. 2010 Aug 26;116(8):1321-8. doi: 10.1182/blood-2009-07-229450. Epub 2010 Apr 21. 10.1182/blood-2009-07-229450 PubMed 20410507
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