pmCherry-ATG5-K130R
(Plasmid #13096)

• Depositing Lab: Roberta Gottlieb
• Publication: Hamacher-Brady et al Cell Death Differ. 2006 Apr 28. ():.

Backbone

• Vector backbone: pEGFPC1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3941
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATG5-K130R
• Alt name: ATG5
• Species: M. musculus (mouse)
• Insert Size (bp): 1576
• Mutation: K130R
• GenBank ID: AB048349
• Entrez Gene: Atg5 (a.k.a. 2010107M05Rik, 3110067M24Rik, Apg5l, Atg5l, Paddy)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The fusion marker mCherry is from Roger Tsien at UCSD
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

for mCherry marker protein
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, Tsien RY. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82