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pLVH-EF1a-GFP-P2A-OCT4
(Plasmid #130692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVH-EF1a-GFP-P2A
  • Total vector size (bp) 8317
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human OCT4
  • Alt name
    POU5F1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1080
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We extensively modified the plasmid of pLVX-AcGFP-C1 (Clontech, 632155) by replacing CMV promoter with EF1a promoter, adding P2A to mediate co-expression of the gene of interest, and removal of PGK promoter and puromycin resistant gene to make a lentiviral backbone vector of pLVH-EF1a-P2A.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVH-EF1a-GFP-P2A-OCT4 was a gift from Kejin Hu (Addgene plasmid # 130692 ; http://n2t.net/addgene:130692 ; RRID:Addgene_130692)

  • For your References section:

    The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Shao Z, Zhang R, Khodadadi-Jamayran A, Chen B, Crowley MR, Festok MA, Crossman DK, Townes TM, Hu K. Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869. 10.1038/ncomms10869 PubMed 26947130
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