28 pCS plcg 1
(Plasmid #13059)

• Depositing Lab: Nathan Lawson
• Publication: Lawson et al Genes Dev. 2003 Jun 1. 17(11):1346-51.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4100

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: plcg1
• Species: D. rerio (zebrafish)
• Insert Size (bp): 3938
• Entrez Gene: plcg1 (a.k.a. fj46f04, wu:fj46f04, wu:fj57a05)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Sp6
• 3′ sequencing primer: EBV rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS. Additional plcg1 cDNA sequence was obtained or confirmed by cycle sequencing of reverse transcriptase PCR (RT