-
Purpose(Empty Backbone)
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13056 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepGT2
- Backbone size (bp) 3267
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XcmI, HindIII, StyI, BspEI, EcoRV (not destroyed)
- 3′ cloning site XcmI, SacI, SpeI, NotI, EcoRI, AatII (not destroyed)
- 5′ sequencing primer M13/pUC Reverse (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byModified from pGFPTA (Husimi et al, 2000)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pGT2 is an XcmI generated T-vector bearing GFP marker optimized for direct cloning.
Colonies with insert are selected by GFP inactivation.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGT2 was a gift from Chaoyang Zeng (Addgene plasmid # 13056 ; http://n2t.net/addgene:13056 ; RRID:Addgene_13056) -
For your References section:
Construction of an XcmI-generated T vector bearing green fluorescent protein marker for direct cloning of PCR products. Park HK, Zeng C. Anal Biochem. 2007 Jan 1;360(1):144-5. doi: 10.1016/j.ab.2006.10.031. Epub 2006 Nov 7. 10.1016/j.ab.2006.10.031 PubMed 17113027
Map uploaded by the depositor.
