pES E38A
(Plasmid
#13049)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13049 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMutS
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SpeciesE. coli
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Insert Size (bp)1848
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Mutationmutation Glu38 to Ala
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Tag
/ Fusion Protein
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Derived from pTX412
Feng, G. and Winkler, M.E.
1995 Biotechniques 19:956
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pES E38A was a gift from Peggy Hsieh (Addgene plasmid # 13049 ; http://n2t.net/addgene:13049 ; RRID:Addgene_13049) -
For your References section:
The Phe-X-Glu DNA binding motif of MutS. The role of hydrogen bonding in mismatch recognition. Schofield MJ, Brownewell FE, Nayak S, Du C, Kool ET, Hsieh P. J Biol Chem. 2001 Dec 7. 276(49):45505-8. 10.1074/jbc.C100449200 PubMed 11602569