pCFD3-Act5c
(Plasmid #130278)

• Purpose: Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.
• Depositing Lab: Norbert Perrimon
• Publication: Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.302819. doi: 10.1534/genetics.119.302819.

Backbone

• Vector backbone: pCFD3
• Vector type: Insect Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pCFD3-Act5c
• Alt name: GP07595
• Species: D. melanogaster (fly)
• Entrez Gene: Act5C (a.k.a. Dmel_CG4027, A, A4V404_DROME, ACT, ACT1_DROME, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, ArpA, BAP47, Bap47, CG4027, Dmel\CG4027, F-ACTIN, F-Actin, F-actin, G-actin, M32055, T11, act, act 5C, act5C, actin, actin5C, actomyosin, anon-EST:fe2D2, beta-Actin, beta-actin, beta-actin/Bap47, chrX:5748184..5748304, cyt5C, f-actin, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pCFD3-Act5c.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a mixed nucleotide population for a single bp in vermillion. If the plasmid contains a C, there is a S322P SNP in vermillion. Depositor confirms this does not affect plasmid function.