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Addgene

pAK_Tol2_TRE_Puro
(Plasmid #130259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pminiTol2
  • Backbone size (bp) 6272
  • Modifications to backbone
    Altered restriction enzyme sites and inserted TRE Promoter with rTetR-Puro
  • Vector type
    Mammalian Expression ; Tol2 Transposon, Doxycycline Inducible
  • Promoter TRE Promoter
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

All-in-one Tol2 transposon system for Dox-On inducible gene expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAK_Tol2_TRE_Puro was a gift from Michael Yaffe (Addgene plasmid # 130259 ; http://n2t.net/addgene:130259 ; RRID:Addgene_130259)
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