WFS1 P724L-Flag-pcDNA3
(Plasmid #13012)

• Depositing Lab: Fumihiko Urano
• Publication: Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-15.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Wolfram Syndrome 1
• Species: H. sapiens (human)
• Insert Size (bp): 2800
• Mutation: changed P 724 to L
• Entrez Gene: WFS1 (a.k.a. CTRCT41, WFRS, WFS, WFSL)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry