pGD283
(Plasmid
#129733)
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Purpose(Empty Backbone) Destination plasmid containing the 35S:XVE estradiol inducible driver and the A-G overhangs for GreenGate cloning of an expression casette for estradiole-inducible expression in plants.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129733 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGGZ001
- Backbone size (bp) 3000
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Modifications to backboneInserted p35S::XVE:trbcS in place of the ccdB cassette and recreated an A-Z GreenGate cloning site 3' of the insert
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/734616v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGD283 was a gift from Rüdiger Simon (Addgene plasmid # 129733 ; http://n2t.net/addgene:129733 ; RRID:Addgene_129733) -
For your References section:
Over the rainbow: A practical guide for fluorescent protein selection in plant FRET experiments. Denay G, Schultz P, Hansch S, Weidtkamp-Peters S, Simon R. Plant Direct. 2019 Dec 6;3(12):e00189. doi: 10.1002/pld3.189. eCollection 2019 Dec. 10.1002/pld3.189 PubMed 31844834
