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Addgene

rpoDs366C
(Plasmid #129688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rpoD
  • Alt name
    Sigma 70
  • Species
    E. coli
  • Mutation
    C132S C291S C295S S366C
  • Entrez Gene
    rpoD (a.k.a. b3067, ECK3057, alt)
  • Tag / Fusion Protein
    • His6/thrombin (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS found a N149D amino acid variation in the rpoD translation, that the depositing laboratory confirms to be a naturally occurring sequence variant. This will not adversely affect plasmid function.

Please visit https://www.biorxiv.org/content/10.1101/2020.02.17.953182v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rpoDs366C was a gift from Jeff Gelles (Addgene plasmid # 129688 ; http://n2t.net/addgene:129688 ; RRID:Addgene_129688)

  • For your References section:

    Mechanism of upstream promoter element stimulation of transcription at a ribosomal RNA promoter determined by single-molecule imaging. Mumm JP, Friedman LJ, Gelles J. bioRxiv 2020 10.1101/2020.02.17.953182
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