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Addgene

pB-Gaolf-Integration
(Plasmid #129456)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129456 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pB-CMV expression vector
  • Backbone manufacturer
    SBI
  • Backbone size w/o insert (bp) 6969
  • Total vector size (bp) 8115
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G alpha olf
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1146
  • GenBank ID
    14680
  • Entrez Gene
    Gnal (a.k.a. 2610011C15Rik, 9630020G10Rik, Galphaolf, Gna10, Golf)
  • Promoter TRE (Tet-On)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGAAGACACCGGGACCGAT
  • 3′ sequencing primer tagcagacactctatgcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-Gaolf-Integration was a gift from Sriram Kosuri (Addgene plasmid # 129456 ; http://n2t.net/addgene:129456 ; RRID:Addgene_129456)

  • For your References section:

    A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing. Jones EM, Jajoo R, Cancilla D, Lubock NB, Wang J, Satyadi M, Cheung R, de March C, Bloom JS, Matsunami H, Kosuri S. Cell Syst. 2019 Mar 27;8(3):254-260.e6. doi: 10.1016/j.cels.2019.02.009. Epub 2019 Mar 20. 10.1016/j.cels.2019.02.009 PubMed 30904378
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