pETM6_Pfrm_BsMdh_BmHps_BmPhi
(Plasmid
#129109)
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PurposeExpresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepETM6
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBsMDH BmHPS BmPHI
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GenBank IDBSU_29120, PB1_00480, PB1_00485
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETM6_Pfrm_BsMdh_BmHps_BmPhi was a gift from Eleftherios Terry Papoutsakis (Addgene plasmid # 129109 ; http://n2t.net/addgene:129109 ; RRID:Addgene_129109) -
For your References section:
Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol. Rohlhill J, Sandoval NR, Papoutsakis ET. ACS Synth Biol. 2017 Aug 18;6(8):1584-1595. doi: 10.1021/acssynbio.7b00114. Epub 2017 May 9. 10.1021/acssynbio.7b00114 PubMed 28463494