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Purpose(Empty Backbone) Module cloning vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128859 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
- Backbone size (bp) 2710
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Modifications to backboneBsaI site destroy in ampicillin cassette
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/630277v1 for BioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC57BsaI was a gift from Gilles Charvin (Addgene plasmid # 128859 ; http://n2t.net/addgene:128859 ; RRID:Addgene_128859) -
For your References section:
COSPLAY: An expandable toolbox for combinatorial and swift generation of expression plasmids in yeast. Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G. PLoS One. 2019 Aug 28;14(8):e0220694. doi: 10.1371/journal.pone.0220694. eCollection 2019. 10.1371/journal.pone.0220694 PubMed 31461461