Ub-GyrA
(Plasmid #128737)

• Purpose: Expresses Ub fused to GyrA intein at the C-terminal
• Depositing Lab: Sharon Rozovsky
• Publication: Liu et al Chembiochem. 2019 Jul 2. doi: 10.1002/cbic.201900160.

Backbone

• Vector backbone: pTWIN1
• Backbone size w/o insert (bp): 7375
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UBC
• Promoter: T7
• Tag / Fusion Protein:
  • Mxe GyrA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: SapI (unknown if destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA