pCPP5371
(Plasmid
#128732)
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Purpose(Empty Backbone) Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128732 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBBR1MCS
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Gentamicin, 25 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCPP5371 was a gift from Alan Collmer (Addgene plasmid # 128732 ; http://n2t.net/addgene:128732 ; RRID:Addgene_128732) -
For your References section:
Pseudomonas syringae lytic transglycosylases coregulated with the type III secretion system contribute to the translocation of effector proteins into plant cells. Oh HS, Kvitko BH, Morello JE, Collmer A. J Bacteriol. 2007 Nov;189(22):8277-89. Epub 2007 Sep 7. 10.1128/JB.00998-07 PubMed 17827286
