pCPP5060
(Plasmid
#128716)
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PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128716 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR201
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameshcN-hopN1
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SpeciesPseudomonas syringae
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also reference: PMID 29742421.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCPP5060 was a gift from Alan Collmer (Addgene plasmid # 128716 ; http://n2t.net/addgene:128716 ; RRID:Addgene_128716) -
For your References section:
HopPtoN is a Pseudomonas syringae Hrp (type III secretion system) cysteine protease effector that suppresses pathogen-induced necrosis associated with both compatible and incompatible plant interactions. Lopez-Solanilla E, Bronstein PA, Schneider AR, Collmer A. Mol Microbiol. 2004 Oct;54(2):353-65. doi: 10.1111/j.1365-2958.2004.04285.x. 10.1111/j.1365-2958.2004.04285.x PubMed 15469508