pRSF-1b-S2
(Plasmid
#128590)
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PurposeExpresses tagless S2 ribosomal protein in E.coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128590 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSF-1b
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name30S ribosomal subunit protein S2
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Alt namerpsB
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SpeciesE.coli
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Insert Size (bp)710
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Entrez GenerpsB (a.k.a. b0169, ECK0168)
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSF-1b-S2 was a gift from James Williamson (Addgene plasmid # 128590 ; http://n2t.net/addgene:128590 ; RRID:Addgene_128590) -
For your References section:
Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA Folding. Duss O, Stepanyuk GA, Puglisi JD, Williamson JR. Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 10.1016/j.cell.2019.10.035. Epub 2019 Nov 21. 10.1016/j.cell.2019.10.035 PubMed 31761533
