pMC037-HisMS2_PLP_pac
(Plasmid #128233)

• Purpose: Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.
• Depositing Lab: Paul Freemont
• Publication: de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111/mmi.14406.

Backbone

• Vector backbone: pMX
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 2200
• Total vector size (bp): 4630
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Maturation Protein
• Alt name: A-Protein
• Species: MS2 Phage
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: GATGTGCTGCAAGGCGATTAAGTTG
• 3′ sequencing primer: ctatggaaaaacgccagcaacg

Gene/Insert 2

• Gene/Insert name: Coat Protein Dimer
• Species: Synthetic
• Insert Size (bp): 801
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: GATGTGCTGCAAGGCGATTAAGTTG
• 3′ sequencing primer: ctatggaaaaacgccagcaacg

Resource Information

• Supplemental Documents:
  • pmc037-hisms2_plp_pac.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA