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Purpose(Empty Backbone) Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128059 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH-EF1-IRES cop GFP
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS analysis identified 3 lentiviral ITR sequences. Depositing lab has indicated that this plasmid is functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-EF1-IRES eGFP was a gift from Oskar Laur (Addgene plasmid # 128059 ; http://n2t.net/addgene:128059 ; RRID:Addgene_128059)