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Oskar Laur Lab: Emory Custom Cloning Core Plasmids - Oskar Laur

Unpublished

Plasmids from Article

ID Plasmid Purpose
128023pCDNA3- c-terminal 6xMycMammalian expression vector with C terminal 6xMyc tag
128024pcDNA3-c-terminal mouseFcMammalian expression vector with C terminal mFC tag
128025pcDNA3-C-terminal GSTMammalian expression vector with C terminal GST tag
128026pcDNA3-C-terminal CyPetMammalian expression vector with C terminal CyPet tag
128027pcDNA3-C-terminal YPetMammalian expression vector with C terminal YPet tag
128028pcDNA3-IL-2 signalMammalian expression vector with N terminal IL-2 signal peptide
128029pcDNA3-IL-2 signal_CyPetMammalian expression vector with N terminal IL-2 signal peptide and CyPet
128030pcDNA3-IL- signal_YPetMammalian expression vector with N terminal IL-2 signal peptide and YPet
128031pcDNA3-IL-2 signal_rabbit FcMammalian expression vector with N terminal IL-2 signal peptide and rabbit Fc
128032pcDNA3-IL-2 signal_GSTMammalian expression vector with N terminal IL-2 signal peptide and GST
128033pcDNA3-IL-2signal-6xMycMammalian expression vector with N terminal IL-2 signal peptide and 6xMyc tag
128034pcDNA3.1-HAMammalian expression vector with N terminal HA tag
128035pVL1393-6xMycBaculovirus expression vector with C-terminal 6xMyc tag
128036pShuttle-CMV-IRES-GFPAdenoviral shuttle vector with CMV promoter, coexpresses EGFP
128037pBabe puro IRES-EGFPRetroviral vector with IRES-EGFP
128038pBabe puro IRES-mCherryRetroviral vector with IRES-mCherry
128040pcDNA3 DSred express2Mammalian expression vector with N terminal DsRed express2
128041 pBABE puro EGFP Retroviral vector with N terminal EGFP
128042pCDNA3 IL-2 signal mFcMammalian expression vector with C terminal IL-2 signal peptide and mFc
128043pMA2780 lenti tet on EGFPTet inducible Lentiviral vector with EGFP co-expression
128044pLEX-MCS PGK promoterLentiviral vector with PGK promoter
128045pLEX-MCS UBC promoterLentiviral vector with UBC promoter
128046pGL3basic luciferase and renilla_polyApGL3 basic luciferase reporter with co-expression of renilla luciferase
128047pcDNA3.1 acGFPMammalian expression vector with N terminal AcGFP
128048pLEX MCS snap tagLentiviral vector with snap tag
128049pShuttle-CMV-GFP U6promoter Adenoviral shuttle vector for shRNA expression from U6 promoter with GFP marker
128050pShuttle-CMV-GFP H1promoter Adenoviral shuttle vector for shRNA expression from H1 promoter with GFP marker
128051pLex MCS mCherry MycLentiviral vector with mCherry Myc
128052prs316 CUP-GFPYeast expression vector for N terminal GFP fusions
128053pGL3 basic EGFPEGFP reporter vector with restriction sites to clone in a promoter of interest, derived from pGL3 basic
128054pGL3 promoter EGFPEGFP reporter vector with restriction sites to clone in an enhancer of interest, derived from pGL3 promoter
128055plenti guide ds redExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.
128057pcdna3.1 hygro mCherryMammalian expression vector with N terminal mCherry
128058pFastbac1 Nterminal GSTBaculovirus expression vector with N terminal GST
128059pCDH-EF1-IRES eGFPLentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.
128060pScarlet-N1Mammalian expression vector for C terminal mScarlet-I fusions
128061pLVX-Tet3G blasticidinLentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.
128062pLenti6-V5-Topo mCherryLentiviral vector for C terminal mCherry fusions
128063plenti guide Thy1.1Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.
128064BS II KS+ loxP-GFP_ZEO_loxPhas loxp-GFP-Zeo-Loxp cassette
128065pShuttle-CMV-GFPAdenoviral shuttle vector with CMV promoter-EGFP
128067FUGW ds redLentiviral expression of dsRed Express2
128068FUGW ECFPLentiviral expression of ECFP
128069pREVtre GFPRetroviral tet inducible EGFP
128070pLVX TRE 3G BFPLentiviral expression of Tet inducible TagBFP
128071FUGW mplum3rd gen lentiviral plasmid with hUbC-driven mPlum
128072pCDH-EF1-IRES plumgfp was exchanged to plum
128073pLKO.1 mCherry puro exchanged to mCherry
128074FUGW-EYFP 3rd gen lentiviral plasmid with hUbC-driven EYFP
129427Lenti puro EF2 LUC-2A-Dendra2expression of LUC and Dendra2 reporters under EF2 promoter
129428pLOC CMV-IRES-Dsred-blasticidinexpression of Dsred-blasticidin under CMVpromoter -IRES
129429plex.mcs sv40promoter-lucLuc expression under SV40 promoter
129431EF1a mCherryexpression of mCherry from EF1a promoter
129432EF1a CHR2 mCherry WPREexpression of CHR2 mCherry from EF1a promoter
129433pRS426 TEF mCherryexpress mcherry
129435pTRE2 EGFPexpress GFP
129436pCDH-UBC-LUC-EF1 Hygroexpress LUC
129437pCDH-UBC-DSRED-LUC-EF1 Hygroexpress dsred-luc chimera
129438pCDH-UBC-irfp luc-EF1 Hygroexpress irfp-luc chimera
129439pCDH-UBC-dendra 2LUC-EF1 Hygroexpress dendra2-luc chimera
129440pCDH-CMV-mCherry-EF1 Hygroexpress mCherry
129441pGEX-4T1 NLS-GFPbacterial expression of GFP
129442pTXB1 EGFPexpression of intein/ gfp
129443EF1a- gfp-2a-puroexpression of GFP and Puro R
129444pCDNA3.3 CAS9 IRES-Dsredexpression of FnCas9 and DsRed
129445pET28a HAT epitopeexpression of HAT epitope

Antibodies from Article