pBirA-P19
(Plasmid
#127919)
-
PurposeTransient expression of biotin ligase BirA and p19 in plant protoplast
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 127919 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMD19-T
-
Vector typePlant Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameBirA
- Promoter 35S
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site PstI (not destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
P19 from pEAQ-HT-DEST1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBirA-P19 was a gift from Silin Zhong (Addgene plasmid # 127919 ; http://n2t.net/addgene:127919 ; RRID:Addgene_127919) -
For your References section:
Reconstructing the maize leaf regulatory network using ChIP-seq data of 104 transcription factors. Tu X, Mejia-Guerra MK, Valdes Franco JA, Tzeng D, Chu PY, Shen W, Wei Y, Dai X, Li P, Buckler ES, Zhong S. Nat Commun. 2020 Oct 9;11(1):5089. doi: 10.1038/s41467-020-18832-8. 10.1038/s41467-020-18832-8 PubMed 33037196