pX330 Mouse 3' Hp1a gRNA
(Plasmid
#127898)
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PurposeWT Cas9 Vector targeting the 3' end of the mouse Hp1a gene
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepX330
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Modifications to backbonegRNA to mouse Hp1a cloned in
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegRNA/Cas9
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gRNA/shRNA sequencegcgcgaagagctaaaggagg
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (destroyed during cloning)
- 3′ cloning site BbsI (destroyed during cloning)
- 5′ sequencing primer cacctctgacttgagcgtcg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byFrom https://www.addgene.org/42230/
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pX330 Mouse 3' Hp1a gRNA was a gift from Mark Groudine (Addgene plasmid # 127898 ; http://n2t.net/addgene:127898 ; RRID:Addgene_127898)
