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Addgene

pLEP1-H-Hsp82
(Plasmid #1277)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1277 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS314
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    his-Hsp82
  • Alt name
    Hsp82
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    HSP82 (a.k.a. YPL240C, HSP90)
  • Tag / Fusion Protein
    • Histidinex6 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEP1-H-Hsp82 was a gift from Susan Lindquist (Addgene plasmid # 1277 ; http://n2t.net/addgene:1277 ; RRID:Addgene_1277)

  • For your References section:

    Conservation of Hsp90 macromolecular complexes in Saccharomyces cerevisiae. Chang HC, Lindquist S. J Biol Chem 1994 Oct 7;269(40):24983-8. PubMed 7929182
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