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Addgene

pKAT6
(Plasmid #1215)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1215 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    YEp24
  • Backbone size w/o insert (bp) 13200
  • Vector type
    Yeast Expression ; 2 micron

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    MC1066
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hsc82
  • Alt name
    hsp90
  • Alt name
    heat shock
  • Alt name
    hsc82
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    vector backbone is YEp24 URA3 selection
  • GenBank ID
    NC_001145
  • Entrez Gene
    HSC82 (a.k.a. YMR186W, HSP90)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI/BglII (destroyed during cloning)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer pBRrevBam
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKAT6 was a gift from Susan Lindquist (Addgene plasmid # 1215 ; http://n2t.net/addgene:1215 ; RRID:Addgene_1215)
  • For your References section:

    Mutational analysis of Hsp90 function: interactions with a steroid receptor and a protein kinase. Nathan DF, Lindquist S. Mol Cell Biol 1995 Jul;15(7):3917-25. PubMed 7791797