pKAT6
(Plasmid
#1215)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1215 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneYEp24
- Backbone size w/o insert (bp) 13200
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Vector typeYeast Expression ; 2 micron
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Unknown
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Growth instructionsMC1066
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHsc82
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Alt namehsp90
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Alt nameheat shock
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Alt namehsc82
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SpeciesS. cerevisiae (budding yeast)
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Mutationvector backbone is YEp24 URA3 selection
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GenBank IDNC_001145
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Entrez GeneHSC82 (a.k.a. YMR186W, HSP90)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI/BglII (destroyed during cloning)
- 3′ cloning site SphI (not destroyed)
- 5′ sequencing primer pBRrevBam (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKAT6 was a gift from Susan Lindquist (Addgene plasmid # 1215 ; http://n2t.net/addgene:1215 ; RRID:Addgene_1215) -
For your References section:
Mutational analysis of Hsp90 function: interactions with a steroid receptor and a protein kinase. Nathan DF, Lindquist S. Mol Cell Biol 1995 Jul;15(7):3917-25. PubMed 7791797