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pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP
(Plasmid #127560)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127560 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRISPaint-T2A-Gal4-3xP3-RFP digested with NheI/KpnI
  • Backbone manufacturer
    Justin Bosch and Norbert Perrimon
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-T2A-GFP
  • Alt name
    T2A-Cas9-T2A-GFP
  • Mutation
    EGFP-D102G see depositor comments below

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAG
  • 3′ sequencing primer GGAAAGTCCTTGGGGTCTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutation in EGFP does not affect expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP was a gift from Norbert Perrimon (Addgene plasmid # 127560 ; http://n2t.net/addgene:127560 ; RRID:Addgene_127560)

  • For your References section:

    Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids. Bosch JA, Colbeth R, Zirin J, Perrimon N. Genetics. 2019 Nov 4. pii: genetics.119.302819. doi: 10.1534/genetics.119.302819. 10.1534/genetics.119.302819 PubMed 31685521
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