R4pGWB601_UBQ10p-Turbo-YFP-NLS
(Plasmid #127368)

• Purpose: Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants
• Depositing Lab: Dominique Bergmann
• Publication: Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864.

Backbone

• Vector backbone: R4pGWB601
• Backbone manufacturer: http://shimane-u.org/nakagawa/gbv.htm
• Total vector size (bp): 12866
• Vector type: Plant Expression
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TurboID (BirA mutant)
• Species: BirA from E. coli, codon optimized for human and mutated
• Insert Size (bp): 957
• Mutation: Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, M241T, S263P, I305V
• Promoter: Arabidopsis UBQ10 promoter
• Tags / Fusion Proteins:
  • V5 (N terminal on insert)
  • GS linker (C terminal on insert)
  • YFP (C terminal on insert)
  • NLS (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 fw
• 3′ sequencing primer: M13 rv

Resource Information

• Supplemental Documents:
  • R4pGWB601_UBQ10p-Turbo-YFP-NLS.gb
• A portion of this plasmid was derived from a plasmid made by: TurboID was obtained from Alice Ting's lab at Stanford University (cloned from V5-hBirA-Turbo-NES_pCDNA3, Addgene catalog #107169).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry