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6MIW
(Plasmid #127291)

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Item Catalog # Description Quantity Price (USD)
Plasmid 127291 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid # 26096)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HUWE1
  • Alt name
    HUWE1:Q1611-L1700
  • Alt name
    SGC Clone Sample ID: HUWE1:YTC050-G10:C241700
  • Alt name
    PDB: 6MIW, 6PFL, 8RD7, 8R7O, 8RD0, 8RD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    270
  • Entrez Gene
    HUWE1 (a.k.a. ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MRXST, MULE, URE-B1, UREB1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-TEV (MHHHHHHSSGRENLYFQG) (N terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6MIW was a gift from Cheryl Arrowsmith (Addgene plasmid # 127291 ; http://n2t.net/addgene:127291 ; RRID:Addgene_127291)

  • For your References section:

    A ligand discovery toolbox for the WWE domain family of human E3 ligases. Munzker L, Kimani SW, Fowkes MM, Dong A, Zheng H, Li Y, Dasovich M, Zak KM, Leung AKL, Elkins JM, Kessler D, Arrowsmith CH, Halabelian L, Bottcher J. Commun Biol. 2024 Jul 24;7(1):901. doi: 10.1038/s42003-024-06584-w. 10.1038/s42003-024-06584-w PubMed 39048679
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