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Addgene

pET51b(+)-SNAP-HCA_sensor
(Plasmid #127181)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127181 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET51-b(+)
  • Backbone manufacturer
    Novagen
  • Total vector size (bp) 8053
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
  • Species
    Synthetic
  • Insert Size (bp)
    2991
  • Promoter T7
  • Tags / Fusion Proteins
    • StrepTag (N terminal on insert)
    • HisTag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET51b(+)-SNAP-HCA_sensor was a gift from Kai Johnsson (Addgene plasmid # 127181 ; http://n2t.net/addgene:127181 ; RRID:Addgene_127181)

  • For your References section:

    Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins. Scarabelli S, Tan KT, Griss R, Hovius R, D'Alessandro PL, Vorherr T, Johnsson K. ACS Sens. 2017 Aug 25;2(8):1191-1197. doi: 10.1021/acssensors.7b00331. Epub 2017 Aug 2. 10.1021/acssensors.7b00331 PubMed 28766337
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