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Addgene

AbVec2.0-mIghg1
(Plasmid #127158)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 5729
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ighg1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    972
  • GenBank ID
    LR588429
  • Entrez Gene
    Ighg1 (a.k.a. IgG1, Igh-4, VH7183)
  • Promoter hCMV IE1 gene promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site BclI (destroyed during cloning)
  • 5′ sequencing primer GCTTCGTTAGAACGCGGCTAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AbVec2.0-mIghg1 was a gift from Hedda Wardemann (Addgene plasmid # 127158 ; http://n2t.net/addgene:127158 ; RRID:Addgene_127158)
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