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px458_2A_GFP_sgRNA_TRIM25_G1
(Plasmid #127119)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127119 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    px458_2A_GFP_sgRNA
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA TRIM25
  • gRNA/shRNA sequence
    GCAGCTACAACAAGAATACA
  • Species
    H. sapiens (human)
  • Entrez Gene
    TRIM25 (a.k.a. EFP, RNF147, Z147, ZNF147)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    px458_2A_GFP_sgRNA_TRIM25_G1 was a gift from Thomas Tuschl (Addgene plasmid # 127119 ; http://n2t.net/addgene:127119 ; RRID:Addgene_127119)

  • For your References section:

    The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation. Meyer C, Garzia A, Morozov P, Molina H, Tuschl T. Mol Cell. 2020 Mar 19;77(6):1193-1205.e5. doi: 10.1016/j.molcel.2019.12.024. Epub 2020 Jan 24. 10.1016/j.molcel.2019.12.024 PubMed 31981475
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