Skip to main content
Addgene

pRSFDuet1-Cdc45
(Plasmid #126878)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 126878 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRSFDuet1
  • Backbone size w/o insert (bp) 3829
  • Total vector size (bp) 5476
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDC45
  • Alt name
    Cell Division Cycle 45
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1723
  • Entrez Gene
    CDC45 (a.k.a. CDC45L, CDC45L2, MGORS7, PORC-PI-1)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • No tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TTGTACACGGCCGCATAATC
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDuet1-Cdc45 was a gift from Luca Pellegrini (Addgene plasmid # 126878 ; http://n2t.net/addgene:126878 ; RRID:Addgene_126878)

  • For your References section:

    Structure of human Cdc45 and implications for CMG helicase function. Simon AC, Sannino V, Costanzo V, Pellegrini L. Nat Commun. 2016 May 18;7:11638. doi: 10.1038/ncomms11638. 10.1038/ncomms11638 PubMed 27189187
Related items:
Commonly requested with: