pET-22b(+)-RNase HIII
(Plasmid
#12683)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12683 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-22b(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5493
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRNase HIII
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SpeciesBacillus subtilis
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Insert Size (bp)939
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GenBank IDBSU28620
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the His tag is not in frame.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-22b(+)-RNase HIII was a gift from SeWon Suh (Addgene plasmid # 12683 ; http://n2t.net/addgene:12683 ; RRID:Addgene_12683) -
For your References section:
Crystallization and preliminary X-ray crystallographic analysis of RNase HIII from Bacillus subtilis. Kwak JE, Lee JY, Han BW, Moon JH, Sohn SH, Park IS, Kim BG, Suh SW. Acta Crystallogr D Biol Crystallogr. 2001 Mar . 57(Pt 3):438-40. 10.1107/S0907444901000440 PubMed 11223525
