Cav1-mRed
(Plasmid #12681)

• Depositing Lab: Richard Pagano
• Publication: Sharma et al Mol Biol Cell. 2004 Jul . 15(7):3114-22.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: canine caveolin-1
• Alt name: cav1
• Alt name: VIP21
• Species: Canis familiaris
• Insert Size (bp): 1289
• GenBank ID: NM_001003296
• Entrez Gene: CAV1
• Tag / Fusion Protein:
  • mRed (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: canine caveolin1 was cut from Cav1-GFP from Ari Helenius. Monomeric red (mRed or mRFP) was cut from a pRSETB plasmid from Roger Tsien, Howard Hughes Medical Institute
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Caveolin-Nat Cell Biol. 2001 May;3(5):473-83.

mRed-Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82.